5. I am a Class 10 student, my board exams are coming soon. Can I get proper guidance and required help on the topic of parts of a compound microscope?

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First, the specimen must be focused under the low-resolution objective lens by coarse adjustment. Any necessary diagrams can be drawn by viewing from this resolution.

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The microscope must not be disturbed by tilting or shaking it while using it. This bouncing alters the light received by the lens, and the viewing of the object of focus alters.

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Diaphragm: It is a structure located below the stage which controls the intensity of light entering the stage. It is of two types: disc and iris.

Always use a clean slide or clean the slide with proper water and wipe it so that any stains on the slide do not appear under the microscope. This can confuse the person whether the perceived object is a stain or a part of the specimen.

Inclination Joint: It is the point where the pillar meets the arm. The whole microscope can tilt at this inclination joint.

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The specimen must be first focused under the low-resolution lens, then later high-resolution lens must be used to target it furthermore.

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The eyepiece and objective lens must be cleaned by silk cotton and transparent liquid, so that dust accumulated on the lens does not hinder the viewing of the slide or focus.

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The person viewing the microscope should place the lens in a well-lit place so that an adequate amount of light strikes the concave part of the mirror under the stage.

Mirror: A concave mirror on one side and plain mirror, on the other hand, can be seen below the stage to focus the light on the stage for a brighter image of the specimen.

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If you are using an oil immersion lens, the oil should always be used for accurate results. As without the use of oil, the refractive index may be changed, which manipulates the reading of the specimen.

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Nose piece: The body tube has three holes that have places to fit the objective lens. This structure is called the nose piece.

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Clips: The stage, on the side of the specimen holder, contains clips that hold the slides on which samples will be placed.

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Body tube: It is the hollow tube at the upper arm, which has one end of the eyepiece lens, and another term has an objective lens. The body tube is altered using the adjustment knobs.

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Stage: It is the rectangular structure that has a hole in the center that allows the light to pass through it. It is below the eyepiece, and most of the specimens are placed on this stage to examine.

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Eyepiece: It is the lens used on the upper part of the body tube. It usually has a resolution of 10x but can be of 5x or 15x. It is the lens through which the person views the specimens.

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A compound microscope is a type of microscope that uses two sets of lenses to magnify the image under the microscope. It has an objective lens that has a resolution of 4x,10x, 40x, 100x, and an eyepiece of resolution of 10x. The usage of both lenses, compounds or magnifies the image of the object below the objective lens to achieve a resolution of 40x, 100x, 400x, 1000x.

A more unobstructed view of the specimen is obtained when the coarse adjustment knob in the microscope is used. The fine adjustment knob gives an accurate picture of the sample.

When a person is using the coarse adjustment lens, care should be taken that the objective lens does not touch the slide of focus. Sometimes, the coarse adjustment lens, when used rapidly, may crush the specimen to be observed, and precise results cannot be obtained, and  the example and the slide also may be damaged.

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The resolution of the eyepiece remains constant, but the resolution of the objective lens can be changed by rotating the nose piece. Hence, the same microscope can be used to view the object of focus in different magnifications.

The intensity of a wave depends on the amplitude of the wave. Since light is also a wave, its intensity will depend on the amplitude of the wave.

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Students must have thoroughly understood about what is a compound microscope, what are its parts and the working mechanism along with the precautions through the above-shared content.

If you are using a high-resolution lens for a specimen, the fine adjustment knob should only be employed. Do not use the coarse adjustment knob as it cannot give accurate results for viewing smaller details.

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The primary principle used here is the principle of compounding or magnification. The magnification factor of the eyepiece multiplied with that of the objective lens. This principle produces a magnified image.

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Fine adjustment knob: It is a tiny knob that is used for focusing on the slide accurately. This knob can make only small adjustments.

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The person using the lens must first arrange the eyepiece. They must arrange the mirror in a manner that the maximum amount of light reaches at the end of the stage. This ensures that the specimen has adequate light for viewing.

Always use a coverslip on the slide of the specimen. This ensures other particles do not accumulate on the sample and alter the results.

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If you want more resolution on the same specimen, the nose piece is turned into a higher resolution. At this point, only a fine adjustment knob should be used as a coarse adjustment knob can crush the slide.

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Objective lens: It is the lens used on the lower part of the body tube. It is attached to the nose piece. Usually, three or four nose pieces are available, which contain the objective lens of different resolutions like 10x, 40x, 100x.

Some lenses will feature two maximum apertures (shown as 1:4-5.6). This means that, as you zoom, the aperture capability changes. So at the shortest focal ...