Light sheet fluorescencemicroscopy

Global Bioimaging sponsored a 2021 seminar (~48min) by Dr. Hari Schroff that reviews the history of lightsheet microscopy, the various technical implementations, and their strengths & weaknesses: https://www.youtube.com/watch?v=_J4FYR3dEHY

Confocalmicroscopy

Light sheetmicroscopyvs confocal

The UA does not currently have a lightsheet microscope in any of the core facilities. Lightsheet microscopy has a number of advantages over confocal microscopy for certain types of samples, but there are also disadvantages to this technique. There are a quite a few ways to design a lightsheet instrument, all of them usually end up with some compromises, trading one thing for another. It is highly unlikely that just one instrument would be able to satisfy all the research needs on campus.

The UArizona Microscopy Alliance is a volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

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light sheet microscopy中文

Another issue is that a lightsheet microscope can easily generate several terabytes worth of data per day and that data will most likely need post-processing.

A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.