"Collimation" refers to all the optical elements in an instrument being on their designed optical axis. It also refers to the process of adjusting an optical instrument so that all its elements are on that designed axis (in line and parallel). The unconditional aligning of binoculars is a 3-axis collimation, meaning both optical axis that provide stereoscopic vision are aligned parallel with the axis of the hinge used to select various interpupillary distance settings. With regards to a telescope, the term refers to the fact that the optical axis of each optical component should be centered and parallel, so that collimated light emerges from the eyepiece. Most amateur reflector telescopes need to be re-collimated every few years to maintain optimum performance. This can be done by simple visual methods such as looking down the optical assembly with no eyepiece to make sure the components are lined up, by using a Cheshire eyepiece, or with the assistance of a simple laser collimator or autocollimator. Collimation can also be tested using a shearing interferometer, which is often used to test laser collimation.

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"Decollimation" is any mechanism or process which causes a beam with the minimum possible ray divergence to diverge or converge from parallelism. Decollimation may be deliberate for systems reasons, or may be caused by many factors, such as refractive index inhomogeneities, occlusions, scattering, deflection, diffraction, reflection, and refraction. Decollimation must be accounted for to fully treat many systems such as radio, radar, sonar, and optical communications.

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The term collimated may also be applied to particle beams – a collimated particle beam – where typically shielding blocks of high density materials (such as lead, bismuth alloys, etc.) may be used to absorb or block peripheral particles from a desired forward direction, especially a sequence of such absorbing collimators. This method of particle collimation is routinely deployed and is ubiquitous in every particle accelerator complex in the world. An additional method enabling this same forward collimation effect, less well studied, may deploy strategic nuclear polarization (magnetic polarization of nuclei) if the requisite reactions are designed into any given experimental applications.

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Other forms of electromagnetic radiation can also be collimated. In radiology, X-rays are collimated to reduce the volume of the patient's tissue that is irradiated, and to remove stray photons that reduce the quality of the x-ray image ("film fog"). In scintigraphy, a gamma ray collimator is used in front of a detector to allow only photons perpendicular to the surface to be detected.

A collimated beam of light or other electromagnetic radiation has parallel rays, and therefore will spread minimally as it propagates. A perfectly collimated light beam, with no divergence, would not disperse with distance. However, diffraction prevents the creation of any such beam.

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Light can be approximately collimated by a number of processes, for instance by means of a collimator. Perfectly collimated light is sometimes said to be focused at infinity. Thus, as the distance from a point source increases, the spherical wavefronts become flatter and closer to plane waves, which are perfectly collimated.

The word "collimate" comes from the Latin verb collimare, which originated in a misreading of collineare, "to direct in a straight line".