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The MTF is measured by, for example, recording a line grid with the camera. The brightness distribution of such a grid corresponds to a sinusoidal curve. The width of the line pattern (line pairs per millimeter – Lp/mm) describes the so-called spatial frequency. The MTF is a function dependent on the spatial frequency, which is defined as the ratio between the amplitude of the sinusoidal curve on the image, and the original sinusoidal curve. The value is always between 1 (optimal transmission) and 0. In contrast to an optical camera, infrared cameras measure thermal contrast. This is called SRF (Slit Response Function).

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These lightweight lenses are excellent as simple magnifiers, with a much larger distortion-free field than spherical lenses.

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After Bite X-Tech is a bug bite itch device to help relieve itch from insect bites. After Bite Advanced liquid + pressure from the x-tech applicator.

The Modulation Transfer Function (MTF) describes how well a camera can represent the detail contrast of an object in the image.

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There are laser diodes, normally based on gallium nitride (GaN), emitting in the near-ultraviolet region. The available power levels, however, are limited. Some ...

Our experienced application engineers ensure competent and reliable customer service in the field. In addition, Optris works closely with numerous distribution partners worldwide.

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Single-molecule localization microscopy (SMLM) techniques, such as single-molecule tracking (SMT), enable in situ measurements in cells from which data-rich metrics can be extracted. SMT has been successfully applied to a variety of biological questions and model systems, aiming to unravel the spatiotemporal regulation of molecular mechanisms that govern protein function, downstream pathway effects, and cellular function. While powerful, SMLM often suffers from low throughput and illumination inhomogeneity, along with microscope and user-induced technical biases. Due to technical limitations in scaling SMLM techniques, a tradeoff between spatiotemporal resolution and throughput has been made historically, restricting broad application of these technologies. Here we address these limitations using Oblique Line Scan (OLS), a robust single-objective light-sheet based illumination and detection modality that achieves nanoscale spatial resolution and sub-millisecond temporal resolution across a 250 × 190 μm field of view. We demonstrate OLS-enabled SMT on Halo-Tagged proteins in living cells capturing protein motion up to 14 μm2 /s. By exploiting the adaptability of the acquisition frame rate and the improved rejection of out of focus light, we extend the utility of OLS beyond cellular compartments with in-solution SMT (isSMT) for single-molecule measurement of ligand-protein interactions and disruption of protein-protein interactions (PPI). We illustrate the versatility of OLS by showcasing two-color SMT, STORM, and single molecule fluorescence recovery after photobleaching (FRAP). OLS expands the range of SMLM applications and paves the way for robust, high-throughput single-molecule investigations of protein dynamics required for drug screening and systems biology studies, both in cells and in solution.

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Since the SRF also depends on where the measurement is taken within the image section, in the Final Inspection Report for its Precision Line infrared cameras Optris states the values for the center of the image as well as for all four corners. The spatial frequency is 9.8 Lp/mm.