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In addition to the short lifetime of bulbs adding to the long-term cost, they also require specialist disposal due to the high-pressure bulb and mercury content.

Since 2006 when CoolLED introduced the first LED Illumination System, LED technology has really taken off and is quickly surpassing the performance of traditional mercury and metal halide-based illumination options.

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LEDs can be easily controlled. They can be switched on and off with precise microsecond timing, which removes the need for a mechanical shutter and improves the temporal resolution of experiments for capturing high-speed events. LED irradiance can also be modulated electronically in 1% steps (0-100%), removing the need for neutral density filters and protecting samples from phototoxicity and photobleaching.

We have a record of >99% on-time delivery in full against acknowledged delivery dates. Our LED microscope lighting products are reliable, but our support goes beyond the product and we pride ourselves on friendly and expert advice whenever it is needed. You will also be covered by our market-leading warranty, which includes three years as standard for all products and a 12 month in-for-out swap if we cannot fix an issue. For extended cover, we also offer a market-leading warranty extension programme.

At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.

What is microscopes

With a broad spectrum of “white” light from traditional microscopy illumination, the majority of light is unwanted and must be removed using optical filters. As individual LED peaks can be controlled independently, a microscope LED light source can be set to illuminate only the desired wavelength peaks matching a particular fluorophore’s excitation spectrum, increasing the signal to noise ratio and improving image contrast.

LED technology is still evolving, and you can keep up with new developments in microscopy illumination by signing up to our newsletter.

Irradiance declines over the lifetime of a mercury or metal halide bulb, whereas LEDs are stable over time, providing reliable and consistent results. LEDs also offer excellent uniformity over the field of view.

This section is where you can find out why LED microscope Illumination Systems are perfectly suited to fluorescence imaging applications, and how CoolLED LED Illuminators for microscopes have become crucial components of modern life science research and clinical laboratories.

Microscope

LEDs are semiconductor devices which emit photons around a specific peak wavelength when an electrical current is passed through them, and as solid-state electronic devices they have introduced a range of benefits for microscopy illumination:

“I need a broader range for my fluorophores”: Only a few wavelength options existed in 2006, and now the CoolLED pE-4000 has 16, spanning 365 nm-770 nm. This can excite in the UV region and right through to fluorophores at the IR end of the spectrum (which is ideal for imaging thick samples while protecting against phototoxicity). Beyond this, our OEM illumination can offer additional wavelengths. Interestingly, this has even made way for the development of new fluorophores which no longer depend on the emission spectrum of a mercury bulb.

“I don’t have the budget for a new piece of kit; bulbs are a consumable item”: Simple, low cost models are available such as the pE-300lite, which can be purchased under the laboratory’s consumable budget.

If you’re not sure whether a CoolLED Microscope Illumination System is right for you, we can provide a free loan unit for testing, with no obligation to purchase.

When Light Emitting Diode (LED) technology for fluorescence microscopy arrived on the scene, it brought with it a host of benefits over traditional microscopy illumination methods such as mercury and metal halide bulbs.

You can choose from a range of products with a variety of LEDs and different levels of control, from single wavelength models and simple white light, through to the latest systems for advanced research applications.

We achieve this through expert LED assembly and unique active cooling technology, enabling irradiance levels up to five times greater than other LED microscopy illumination systems. By holding the LEDs at a constant temperature through thermal management, we can drive them more powerfully to increase irradiance, stability, reliability and lifetime while offering the lowest energy consumption on the market.

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We are renowned for customer commitment and offer local support through our global network of resellers and our Global Sales Team, which means there is always an expert available to help.

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.

Research and Development efforts have overcome many of the initial limitations and we want to update a few common misconceptions.

LED microscope lighting technology has matured to provide many powerful LEDs covering the full visible spectrum, from the UV across the visible range and into the IR.

Focusing inmicroscope

Bulbs frequently need replacing, whereas LEDs are long-lived. The alignment required after frequently replacing bulbs is tricky, but LEDs can be factory aligned and are ready to be fitted to the microscope.

There’s also the option to work without a liquid light guide, with Direct Fit microscope LED Light Sources offering the most efficient means of light delivery to a microscope. Only CoolLED provides this option, and our most popular type of illumination system harnesses more power whilst keeping total cost to a minimum thanks to fewer consumables.

“LEDs don’t provide enough intensity for fluorescence microscopy”: Intensity, or irradiance, has come a long way from the first system, thanks to CoolLED’s R&D team who are always innovating. And GYR irradiance is improving all the time. It is also important to remember that higher irradiance is not always beneficial, and lower levels protect against photobleaching and phototoxicity.

With the Flex Arm, adjustable mount & Ball-head technology, this mount is designed to provide users with complete versatility when capturing videos & taking images.

Our systems are dedicated to generating bright, high-contrast images to enhance data generation and interpretation for researchers and clinicians.

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.

Please select user typeResellerEnd User Preferences To receive the most relevant information, please let us know what you are interested in:

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

Mercury in bulbs is hazardous to people and the environment. Bulbs are also left on for days during experiments, whereas LEDs can be switched off easily when not in use, which reduces energy consumption. In addition to this, LEDs are more energy efficient and emit much less heat.

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