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Mercury Arc Lamps - The mercury arc lamp remains a workhorse in fluorescence microscopy and is still considered one of the best illumination sources, especially for those fluorophores whose excitation maxima coincide with the spectral lines emitted by the hot mercury plasma.
Halogen Regenerative Cycle - In the halogen regenerative cycle, which operates in tungsten halogen incandescent lamps, vaporized tungsten reacts with hydrogen bromide to form gaseous halides that are subsequently re-deposited onto cooler areas of the filament rather than being slowly accumulated on the inner walls of the envelope. This interactive tutorial demonstrates how halogens combine with tungsten and oxygen to complete the halogen regenerative cycle in incandescent tungsten halogen lamps.
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LED Illumination for Microscopy - Among the most promising of emerging technologies for illumination in optical microscopy is the light-emitting diode (LED). These versatile semiconductor devices possess all of the desirable features that incandescent (tungsten halogen) and arc lamps lack, and are now efficient enough to be powered by low-voltage batteries or relatively inexpensive switchable power supplies. The interactive tutorial featured in this section explores the ZEISS Colibri LED illumination system for widefield fluorescence microscopy.
Fundamentals of Illumination Sources for Optical Microscopy - This discussion addresses brightness, stability, coherence, wavelength distribution, and uniformity in the most common light sources currently employed for investigations in transmitted and fluorescence microscopy.
Infrared heating uses infrared lamps, commonly called heat lamps, to transmit infrared radiation to the body that is being heated. When a body with a large surface area needs to be heated, an array of infrared lamps is often used. The lamp commonly contains an incandescent bulb that produces infrared radiation. Infrared lamps have many industrial applications including curing coatings and preparing plastic for forming, commercial applications such as cooking and browning food, and personal applications such as providing heat (especially in bathrooms and for pets) [6] as well as for commercial and industrial heating.[7]
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Metal Halide Lamps - Metal halide illumination sources are rapidly emerging as a serious challenger to the application of mercury and xenon arc lamps for investigations in fluorescence microscopy. These light sources feature a high-performance arc discharge lamp housed in an elliptical reflector that focuses the output into a liquid light guide for delivery to the microscope.
Light-Emitting Diode Operation - Among the most promising of emerging technologies for illumination in optical microscopy is the light-emitting diode (LED). These versatile semiconductor devices possess all of the desirable features that incandescent (tungsten-halogen) and arc lamps lack, and are now efficient enough to be powered by low-voltage batteries or relatively inexpensive switchable power supplies. This interactive tutorial explores how two dissimilar doped semiconductors can produce light when a voltage is applied to the junction region between the materials.
Infrared lamps are electrical devices which emit infrared radiation. Infrared lamps are commonly used in radiant heating for industrial processes and building heating.[1] Infrared LEDs are used for communication over optical fibers and in remote control devices. Infrared lamps are also used for some night vision devices where visible light would be objectionable. Infrared lamp sources are used in certain scientific and industrial instrument for chemical analysis of liquids and gases; for example, the pollutant sulfur dioxide in air can be measured using its infrared absorption characteristics. IR radiant energy emitted by lamps cover a wide spectrum of wavelengths, ranging from 0.7 μm (micrometers) to a longer wavelength of 400 μm.[2]
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The overall performance of the various illumination sources available for optical microscopy depends on the emission characteristics and geometry of the source, as well as the focal length, magnification and numerical aperture of the collector lens system. These, in turn, are affected by the shape and position of lenses and mirrors within the system. In gauging the suitability of a particular light source, the important parameters are structure (the spatial distribution of light, source geometry, coherence, and alignment), the wavelength distribution, spatial and temporal stability, brightness, and to what degree these various parameters can be controlled.
Incandescent light bulbs use a tungsten filament heated to high temperature to produce visible light and, necessarily, even more infrared radiation. Round bulbs, often tinted red to reduce visible light, provide infrared radiant heat suitable for warming of people or animals, but the power density available is low. The development of quartz halogen linear lamps allowed much higher power density up to 200 watts/inch of lamp (8 w/mm), useful for industrial heating, drying and processing applications.[3] By adjusting the voltage applied to incandescent lamps, the spectrum of the radiated energy can be made to reduce visible light and emphasize infrared energy production. Different wavelengths of infrared radiation are differently absorbed by different materials.[2]
Light-Emitting Diodes (LEDs) - Among the most promising of emerging technologies for illumination in optical microscopy is the light-emitting diode (LED). High-power diodes generate sufficient intensity to provide a useful illumination source for a wide spectrum of applications in fluorescence microscopy.
Coherence of Light - One of the important parameters of illumination sources is their coherence, which is somewhat related to brightness due to the fact that extremely bright light sources are more likely to be highly coherent. This tutorial examines how incoherent light emitted by an arc lamp can be passed through a slit and filter to increase coherence and narrow the wavelength band.
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Microscope lightfunction
Light Source Power Levels - Choosing the appropriate light source for investigations in optical microscopy is highly dependent upon the illumination strategy (transmitted or episcopic), specimen parameters, microscope configuration, and the detector sensitivity.
Mercury Lamphouses - High pressure mercury plasma arc-discharge lamps are highly reliable, produce very high flux densities, and have historically been widely used in fluorescence microscopy. This interactive tutorial examines advanced mercury arc lamphouses that are capable of automatic bulb alignment and intensity control.
Some applications use the heat generated by the standard incandescent lamps, such as incubators, brooding boxes for poultry,[4] heat lights for reptile tanks,[5] novelty lamps such as lava lamps, and the Easy-Bake Oven toy. Heat lamps may have a red coating to reduce the visible light emitted.
Aug 19, 2020 — Put you camera on a tripod and put the ring light on a light stand in front of the camera so that the lens is in the middle of it. Don't try to ...
Elliptical Reflectors - Advanced light sources suitable for use in high-performance fluorescence microscopy couple metal halide arc lamps with elliptical collection mirrors and high-speed filter wheels for rapidly shifting the output wavelength. These sources also provide fiber optics or liquid light guides for coupling the output to the microscope optical train. This interactive tutorial explores how careful positioning of the arc with respect to elliptical reflector focal points is critical to the formation of a focused beam at the input of a liquid light guide.
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Sep 20, 2022 — In fluorescent microscopy techniques, samples are treated with fluorophores so they re-emit light after being excited by a light source. Users ...
20171219 — Thus, only a sheet of light is focused onto the sample. In other words, the sample is illuminated under an angle that is defined by the NA of ...
Microscope lightsource definition
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Xenon Arc Lamps - The xenon arc lamp, which features a largely continuous and uniform spectrum across the entire visible spectral region, is suited to stringent applications requiring the simultaneous excitation of multiple fluorophores over a wide wavelength range in analytical fluorescence microscopy.
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Tungsten-Halogen Lamps - Incandescent tungsten-halogen lamps have been successfully employed as a highly reliable light source in optical microscopy for many decades and continue to be the one of the illumination mechanisms of choice for a variety of imaging modalities.
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Solid-state light emitting diodes can be produced to be efficient sources of near-monochromatic infrared energy. Such sources can be rapidly modulated for communication systems and control signals. A light emitting diode can be closely coupled to an optical fiber, allowing infrared signals to be sent up to scores of kilometres without amplification.
Arc Lamp Instability - Illumination sources based on plasma discharge (arc lamps) require a considerable period after ignition to reach thermal equilibrium, a factor that can affect temporal, spatial, and spectral stability. This tutorial examines several of the origins of arc lamp instability, including wander, flare, and flutter.
IR data transmission is also employed in short-range communication among computer peripherals and personal digital assistants. These devices usually conform to standards published by IrDA, the Infrared Data Association. Remote controls and IrDA devices use infrared light-emitting diodes (LEDs) to emit infrared radiation which is focused by a plastic lens into a narrow beam. The beam is modulated, i.e. switched on and off, to encode the data. The receiver uses a silicon photodiode to convert the infrared radiation to an electric current. It responds only to the rapidly pulsing signal created by the transmitter, and filters out slowly changing infrared radiation from ambient light. Infrared communications are useful for indoor use in areas of high population density. IR does not penetrate walls and so does not interfere with other devices in adjoining rooms. Infrared is the most common way for remote controls to command appliances.
Polarization is a phenomenon that is observed in certain electromagnetic radiations, including light waves. In a polarized light wave, the direction and ...