Differential interference contrast microscopy

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Microscope

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It can be frustrating adjusting your controls each time the objective is changed – especially for critical work. But it quickly becomes second nature.

Criticalillumination

When your microscope is aligned with Kohler illumination properly, the condenser is at the correct height relative to the sample, the field diaphragm is adjusted to eliminate reflections and glare, and the aperture is moved to an optimal position to provide contrast without producing shading artifacts.

Using this method, your images will have much greater clarity and contrast, with little or no glare. And if you’re into microscopy, you’ll be able to meet journal quality standards – in less than a minute of your time!

You’ll find the aperture diaphragm at the bottom of the condenser (also usually controlled by a small level on the side).

科勒照明

To set up for Koehler, head for the (usually neglected) bottom half of your microscope because that’s where you’ll find all the components you’ll need to adjust – two diaphragms and your condenser.

Opticalmicroscope

The field diaphragm controls the area of the circle of light illuminating your specimen, while the aperture diaphragm controls the angular aperture of the cone of light from the condenser.

It’s pretty simple to set up and it should only take a minute or two before you’re using your microscope to its full potential.

Most modern microscopes are capable of using Koehler illumination or are at least capable of being retrofitted as long as certain criteria can be met.

Aligning your microscope for Koehler illumination is easy and will give you clearer images with much better contrast, so you can see (and capture) all the important details.

You’ll find the field diaphragm at the top of your illuminator (it’s usually controlled by a little lever on the side or front).