They have a special ability to cut the glare of light reflected from water or glass (see Figure 1). Polaroids have this ability because of a wave characteristic ...

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Machine vision dome lights provide diffuse lighting, also known as cloudy-day illumination. Reflected light provides non-directional, soft illumination ...

Light-sheet microscopy is a growing area in the field with many different variations being developed and published, due to the enhanced benefits on speed, signal to noise and resolution this modality has over traditional imaging techniques. Put simply, the main difference between traditional imaging modalities and light-sheet microscopy is the decoupling of the excitation light-path from the detection light-path. In traditional imaging modalities (widefield and confocal), excitation light illuminates the sample through the Z-axis and emission light is collected with that same objective, this results in the entire Z-axis being illuminated for each plane of the acquisition. By comparison, light-sheet microscopy works by illuminating a single plan of the sample with a sheet of light, minimizing out of focus light while greatly increasing signal to noise.

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Most light-sheet microscopes have at least two objectives, one for excitation and one to capture emission light, as per the diagram below. Acquisition speed can be sped up by moving the illumination sheet, rather than the sample while spatial resolution can be increased by using thinner illumination light-sheets and/or high-NA collection objectives. There are many different variations on Light-sheet microscope designs to cater for very small samples (e.g. cells) through to larger whole organisms (e.g. Zebrafish), or for very high-speed acquisitions, or very high-resolution systems, but as with most imaging modalities, there is no single system that can achieve everything.

Want to avoid a photo exposure disaster? Don't just wing it, use a light meter for pinpoint accuracy. Your photos will thank you.

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ACRF Cancer Biology Imaging FacilityInstitute for Molecular BioscienceLevel 6N, 306 Carmody Road,Building 80University of Queensland4072, St Lucia,Queensland, Australia

The eyepiece is also known as the ocular lens. The light is made to pass through the thin transparent object. A magnified image of the object is obtained by the ...