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In this technique, a synchrotron light source is used to generate an intense and coherent X-ray beam, which is then focused onto the sample using a specialized objective lens. The objective lens acts as a collimator to select and focus the scattered light, which is then detected by the 2D detector to create a diffraction pattern.[1] The specialized objective lens in DFXM, referred to as an X-ray objective lens, is a crucial component of the instrumentation required for the technique. It can be made from different materials such as beryllium, silicon, and diamond, depending on the specific requirements of the experiment.[8] The objective enables one to enlarge or reduce the spatial resolution and field of view within the sample by varying the number of individual lenses and adjusting p ′ {\displaystyle p'} and q ′ {\displaystyle q'} (as in the figure) correspondingly. The diffraction angle 2 θ {\displaystyle 2\theta } is typically 10–30°.[9][10]

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DFXM has shown potential for imaging biological tissues and organs with high contrast and resolution. It has been used to visualize the 3D microstructure of cartilage and bone, as well as to detect early-stage breast cancer in mouse model.[1][29]

An embedded crystalline element (for example, a grain or domain) of choice (green) is aligned such that the detector is positioned at a Bragg angle that corresponds to a particular diffraction peak of interest, which is determined by the crystal structure of the sample. The objective magnifies the diffracted beam by a factor M = q ′ / p ′ {\displaystyle M=q'/p'} and generates an inverted 2D projection of the grain. Through repeated exposures during a 360° rotation of the element around an axis parallel to the diffraction vector, G {\displaystyle G} , several 2D projections of the grain are obtained from various angles.[12] A 3D map is then obtained by combining these projections using reconstruction algorithms[13] similar to those developed for CT scanning. If the lattice of the crystalline element exhibits an internal orientation spread, this procedure is repeated for a number of sample tilts, indicated by the angles α {\displaystyle \alpha } and β {\displaystyle \beta } .[1]

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The first experimental demonstration of dark-field X-ray microscopy was reported in 2006 by a group at the European Synchrotron Radiation Facility in Grenoble, France. Since then, the technique has been rapidly evolving and has shown great promise in multiscale structural characterization.[1] Its development is largely due to advances in synchrotron X-ray sources, which provide highly collimated and intense beams of X-rays. The development of dark-field X-ray microscopy has been driven by the need for non-destructive imaging of bulk crystalline samples at high resolution, and it continues to be an active area of research today. However, dark-field microscopy,[3][4] dark-field scanning transmission X-ray microscopy,[5] and soft dark-field X-ray microscopy[6] has long been used to map deeply embedded structural elements.

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The sample is positioned at an angle such that the direct beam is blocked by a beam stop or aperture, and the diffracted beams from the sample are allowed to pass through a detector.[11]

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MM1 Owners: Earlier editions of the Stand arrived with a custom spacer for the First Edition (MM1). The current batch does not have this spacer, so if you are going to use your Stand with a First Edition Mini Museum please be sure to let us know so we can include a spacer in your shipment.

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The instrumentation required for DFXM is expensive and typically only available at synchrotron facilities, making it inaccessible to many researchers. Although DFXM can achieve high spatial resolution, it is still not as high as the resolution achieved by other imaging techniques such as transmission electron microscopy (TEM) or X-ray crystallography.[11]

To assist you, you'll find step-by-step instructions below as well as a nice video to walk you through the process. These instructions will also be included in the box, so not to worry if you are giving the Stand as a gift.

The current implementation of DFXM at ID06, ESRF, uses a compound refractive lens (CRL) as the objective, giving spatial resolution of 100 nm and angular resolution of 0.001°.[14][15]

DFXM has been used for the non-destructive investigation of polycrystalline materials and composites, revealing the 3D microstructure,[16] phases,[17] orientation of individual grains,[18][19] and local strains.[20][21] It has also been used for in situ studies of materials recrystallisation,[22] dislocations[23][24] and other defects, and the deformation[20] and fracture mechanisms in materials, such as metals[11] and composites.[25] DFXM can provide insights into the 3D microstructure and deformation of geological materials such as minerals and rocks,[1] and irradiated materials.[26]

c.81 If ambient light is below 300 lux [28 fc], task lights providing 300 to 500 lux [28 to 46 fc] at the work surface are available upon request. Part 2 ...

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MM5 Backers: If you added a Stand to your pledge during the Fifth Edition campaign, please know that we have reserved your items. No need to place a second order! In addition, Stands will also be available in the Pledge Manager very soon.

Preparation of samples for DFXM imaging can be challenging, especially for samples that are not crystalline. There are also limitations on the sample size that can be imaged as the technique works best with thin samples, typically less than 100 microns thick, due to the attenuation of the X-ray beam by thicker samples.[1] DFXM still suffers from long integration times, which can limit its practical applications. This is due to the low flux density of X-rays emitted by synchrotron sources and the high sensitivity required to detect scattered X-rays.[11]

DFXM has the potential to revolutionise the field of nanotechnology by providing non-destructive, high-resolution 3D imaging of nanostructures and nanomaterials. It has been used to investigate the 3D morphology of nanowires and to detect structural defects in nanotubes.[27][28]

Insert your thumbs into the cradle and PUSH the top off. Use gentle, even pressure. You may need to alternate pushing between each thumb.

Three colored gel inserts are included to allow you to easily adjust the color. The color filters are meant to be used under or on top of the Mini Museum. When placed under the Mini Museum, the color effect is very dramatic. When placed on top, it is more subtle. The filters can also be combined to create different colors.

The Micro USB port does NOT charge the batteries. The Illuminated LED Stand uses three (3) standard AAA batteries which are housed in the base and accessed through a panel on the bottom.

The Stand arrives completely assembled and is composed of three (3) simple pieces: the Base, the Cradle, and the Top Cornice. All three parts are injection molded using a PC-ABS blend of durable thermoplastics with a soft, white finish.

The Illuminated LED Stand inside this box is fresh from the factory. It is fully assembled for safe shipping and must be taken apart before use. However, you may find the components are fit together very tightly. This is partially by design to make sure the Stand is secure when assembled with the Mini Museum inside, but it does make the first assembly a little tricky.

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The intense X-ray beams used in DFXM can damage delicate samples, particularly biological specimens.[1] DFXM can suffer from imaging artefacts such as ring artefacts, which can affect image quality and limit interpretation.[11]

Please Note: The Illuminated LED Stand does not come with the Mini Museum. It is an accessory for LARGE Mini Museums only.

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There is a small 3-way switch on the rear of the base, as well as a Micro USB port. The switch is used to select the mode of operation (USB, Battery, or OFF). This allows you to plug the stand into a computer, wall outlet, or run cordless anywhere.

First Edition (MM1) Owners: Early production runs of the Stand arrived with a custom spacer for the First Edition (MM1). The current batch does not have this spacer, so if you are going to use your Stand with a First Edition Mini Museum please be sure to let us know so we can include a spacer in your shipment. We have a limited number of spacers on hand but more are on the way.

The Mini Museum Illuminated LED Stand is designed to highlight the beauty of each Edition, from the First to the Fifth, Age of Dinosaurs, and Beyond!

Dark-field X-ray microscopy (DFXM[1] or DFXRM[2]) is an imaging technique used for multiscale structural characterisation. It is capable of mapping deeply embedded structural elements with nm-resolution using synchrotron X-ray diffraction-based imaging. The technique works by using scattered X-rays to create a high degree of contrast, and by measuring the intensity and spatial distribution of the diffracted beams, it is possible to obtain a three-dimensional map of the sample's structure, orientation, and local strain.