Shaobinggao/Multi-illuminant-based-color-constancy - illmunation
Fluorescencemicroscope
Active illumination microscopy is a simple and straightforward means by which to effectively control and manage the effects of photobleaching and phototoxicity.
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When you fix your tissue samples with paraformaldehyde (PFA) the proteins in your sample become covalently cross-linked. This is good to preserve the ‘architecture’ of your tissue sample. However, this cross-linking can become a problem when you carry out immunohistochemistry (IHC). Cross-linking can ‘mask’ or hide your antigens-of-interest and make them ‘invisible’ to your IHC…
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Type ofmicroscope
Types of Microscopy · Standard bright-field microscopy · Phase contrast microscopy · Fluorescence microscopy · Dark field microscopy · Polarization microscopy.
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
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Dark fieldmicroscopy
I have a Masters of Clinical Research, and a PhD in Molecular & Medical Genetics. However I love keeping up with a wide variety of scientific topics – making my work as a Managing Editor at BitesizeBio very enjoyable.
DIC microscopy
Phase contrast microscopy
How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!
Polarizingmicroscope
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An edge-lit LED panel is made of a row of LEDs attached to the frame (or circumference) of the panel, shining horizontally into a light-guide plate (LGP).
If you’re thinking FRAP is short for frappuccino then you need to read this article. Discover the history, how it works, and why you’d want it in your confocal toolbox
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Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really daunting. Not to mention that no one will ever double check your count if you don’t take a picture. Those…
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For a long time we’ve been able to pinpoint the subcellular location of proteins, and the advent of FISH (Fluorescence in situ Hybridization) allowed us to locate the position of genes in the nucleus, but recent advances in RNA FISH are making it easier and easier to collect the same data about individual messenger RNAs….