Band passfilter circuit

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How to cite this article: Sriram CK, Sivakumar A. Cost-effective method of dark field microscopy in everyday practice. CosmoDerma 2022;2:132.

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Our technique is a simple and cost-effective method and can be used in a resource poor setting. The illumination of a dark ground microscope through this method allows for easy detection of treponemes as compared to the ordinary light microscope thus aiding in early diagnosis and treatment.

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When a specimen is placed on a slide, the oblique rays interact with the specimen and are reflected by it. This allows these faint rays to enter the objective lens. The result is a bright specimen on a dark background aiding in the visualization of the treponema as shown in [Figure 3].

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*Corresponding author: Aravind Sivakumar, Department of Dermatology, Velammal Medical College and Hospital, Madurai, Tamil Nadu, India. aravinddermat@gmail.com

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The dark ground microscope creates a contrast between the object and the surrounding field, such that, the background is dark, and the object is bright. The objective and the ocular lenses used in the dark field microscope are the same as in the ordinary light microscope, with the addition of a special Abbe darkfield condenser or a black annular opaque filter which is used to prevent the transmitted light from directly illuminating the specimen. Only oblique scattered light reaches the specimen and passes onto the lens system causing the object to appear bright against a dark background.[2,3]

Dark-field microscopy is an optical tool that is used in the field of venereology. In contrast to bright-field microscopy, it has the advantage of increasing background contrast to the object thus providing better sensitivity for diagnosis.[1]

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Instead of using a special filter, a one-rupee coin is beneath the condenser as shown in [Figures 1 and 2, Video 1]. The size of the coin must be smaller than that of the condenser such that the rays emerging from the periphery form an inverted hollow cone of light with the focus centered on the specimen plane. Here, the one-rupee coin is selected as its specific diameter of 22 mm allows adequate occlusion while allowing the periphery to be illuminated.

It is the most specific and sensitive technique to diagnose syphilis in the primary stage and can confirm the diagnosis before serological testing. However, dark field microscopes are quite expensive and not widely used. Hence, we are proposing a cost effective novel method to convert a light microscope into a dark field microscope that can be used in resource poor settings.

Knowles DLI brand microstrip bandpass filters offer classical filter topologies yielding excellent performance in a small footprint when fabricated on ceramic substrate materials. Miniaturization can reach new levels by employing DLI high-k ceramic materials. Different techniques are employed to realize small size and high performance. Catalog devices come complete with integrated shielding and the surface mount versions are designed for use on most RF PWB materials. Typical features include center frequency from C to above Ka band although custom devices are avaliable from 1 to 60 GHz. Bandpass filters pass desired frequency range and suppress the rest of undesired spectrum. Designed from 800MHz to 67GHz.