Bright-field microscopy, a method of microscopy in which the object to be observed is located in front of a bright background, called the bright field, which illuminates the microscopic image. In this process, the light passing through the object (transmitted light microscopy) or the light reflected back from it as a whole (reflected light microscopy) enters the objective of the microscope. Bright-field microscopy requires high-contrast objects, so many transmitted-light objects must be stained. In brightfield microscopy, objects appear dark or colored on a light background.

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Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.

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Biodiversity, functioning ecosystems, clean water and intact soils all make up our natural resource base. In the face of global change, employees at the Helmholtz Centre for Environmental Research – UFZ are united by the …

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As a Helmholtz centre for polar and marine research the Alfred Wegener institute works above all in the cold and temperate regions of the world. Together with numerous national and international partners we are involved …

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The Ernst Ruska-Centre (ER-C) for Microscopy and Spectroscopy with Electrons is a national user facility open to universities, research institutions and research laboratories in industry. The ER-C is run conjointly by Forschungszentrum Jülich GmbH and …

The Tescan Tensor STEM is an integrated analytical precession-assisted 4D-STEM instrument for multimodal characterization of nanoscale morphological, chemical, structural and crystallographic properties of a wide range of materials systems. It is equipped with a Schottky …

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DESY is one of the world’s leading accelerator centres. Researchers use the large-scale facilities at DESY to explore the microcosm in all its variety – from the interactions of tiny elementary particles and the behaviour …

Scientists at the Helmholtz Centre for Infection Research (HZI) investigate the mechanisms of infectious diseases and the defence against them. Subsequently, they develop the results of basic research systematically towards medical and pharmaceutical application. The …

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Forschungszentrum Jülich is a large-scale national research facility in the Helmholtz Association of German Research Centres. By conducting research on the global challenges currently facing society, we contribute to the welfare of mankind and responsible …

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Bright-field microscopy, a method of microscopy in which the object to be observed is located in front of a bright background, called the bright field, which illuminates the microscopic image. In this process, the light passing through the object (transmitted light microscopy) or the light reflected back from it as a whole (reflected light microscopy) enters the objective of the microscope. Bright-field microscopy requires high-contrast objects, so many transmitted-light objects must be stained. In brightfield microscopy, objects appear dark or colored on a light background.Resources used"Hellfeldmikroskopie." Lexikon der Optik, Spektrum Akademischer Verlag, Heidelberg, 1999. Web. 3 Jun. 2022.

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The entire field of view appears dark when there is no sample on the microscope stage. However, when a sample is placed on the stage it appears bright against a ...

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The FEI (now ThermoFisher) Titan G3 50-300 PICO is a unique fourth generation transmission electron microscope which has been specifically designed for the investigation of a wide range of solid state phenomena taking place on …

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My name is Riccardo, I am from Italy, living in Leipzig since 2012 and currently working in UFZ. I am actively working in the field of image data management and I am Data Steward in NFDI4BIOIMAGE.

Project Manager for the Helmholtz Transfer Academy "Future Innovators". Interested in anything that can solve problems. Trying to unlock the potential of scientific development to also solve short-term problems of the real-world.

Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.

Biological oceanographer with extensive research experience in mechanistic studies on production, export, transformation, and recycling of organic matter in the ocean. Strong focus on biogeochemistry and microbiology where I use multidisciplinary approaches to link small-scale processes to the full water …

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