The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.

The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.

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TTL & analogue 8 TTL inputs allow independent on/off control of each channel 8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera Triggering speed <7 µs

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In the Lightbridge graphical user interface (see below), live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.

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It is important to note that The LightBridge is only compatible with the pE-800 Series. In addition to The LightBridge, which can be downloaded here, users can also benefit from full integration into third party imaging software.

Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can also be selected.

pE-800 Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg pE-800 Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg

Sequence Runner Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out or other external hardware, and therefore requires minimal electronic hardware.

For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.

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Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.

Diffuse light is necessary for many applications, as soon as reflective, polished, glossy or metallic objects must be tested.

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

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For optimum DAPI excitation, the pE-800 is available with a 365 nm or 400 nm LED for compatibility with existing filter sets. Find out more in our tutorial video here.

Tight hardware synchronisation not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.

The compact pE-800 Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-low power consumption. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800 is a natural choice for cleaner, greener labs.

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We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8  (USB controlled TTL Conversion Kit).

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The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.

Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.

The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

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Software control is achieved through a fast USB 2.0 or RS-232 connection, and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

We are working to fully integrate the pE-800 into all major third party imaging software programs, but in the meantime you can use the pE-6501-8 USB controlled TTL trigger box for software control. Tutorial videos show you how to configure cellSens and MetaMorph.

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.

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Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.

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Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

The pE-800 is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities.

The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).

All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.

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