TTL & analogue 8 TTL inputs allow independent on/off control of each channel 8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera Triggering speed <7 µs

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.

All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.

AIHydra 26

The pE-800 is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities.

At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.

AIHydra 32

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

AIHydra 64

The compact pE-800 Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-low power consumption. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800 is a natural choice for cleaner, greener labs.

Control of the lights will utilize the existing myAI® app, which is free, simple, and very quick to program, offering onsite direct BLE connection through your smart device. Additional control options may become available for the new generation of Prime and Hydras over time.

The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).

The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

AIAquarium

Water resistance is a significant advantage in aquarium lighting. While the new Prime and Hydras are not waterproof, design improvements make them more capable of handling the moisture-rich environments typical in reef keeping.

Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

pE-800 Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg pE-800 Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg

Ai PRIME 16HD freshwater with the latest LED lamp from Ai for your freshwater aquarium. Enormous light in a small housing

Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8  (USB controlled TTL Conversion Kit).

AIPrimelights

Tight hardware synchronisation not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.

The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.

The bracket for the 32HD and 64HD on the flex mount will change in the Flex Arm packaging. However, HMS mounting solutions remain the same, and Prime mounting solutions are also unchanged.

The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.

Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

AI’s popular HD functionality allows users to route unused power from one color channel to another. This much-loved feature from the previous generation HD fixtures is retained in the new product names (Prime 16HD, Hydra 32HD, and 64HD).

It is important to note that The LightBridge is only compatible with the pE-800 Series. In addition to The LightBridge, which can be downloaded here, users can also benefit from full integration into third party imaging software.

This is a Freshwater LED and it is not recommended for Saltwater. Introducing the new Freshwater Prime 16HD series of LED lights.

For optimum DAPI excitation, the pE-800 is available with a 365 nm or 400 nm LED for compatibility with existing filter sets. Find out more in our tutorial video here.

A dedicated moonlight LED and channel have been added in the new Prime HD, Prime FW, Hydra 32HD, and Hydra 64HD modules. This allows for independent moonlighting designed to simulate the natural lunar color and intensity.

AILight

Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.

For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.

Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can also be selected.

Software control is achieved through a fast USB 2.0 or RS-232 connection, and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

Mounting hardware available for Hydra and Prime fixtures will be compatible with the new version lights. The only exception is the Hydra Flex arm, which will require a different light bracket due to size differences in the new fixtures. The Flex arm bracket will change with the new fixtures, and older flex arms will need a new light bracket to mount the new lights.

AIPrime

We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

AIlight app

In the Lightbridge graphical user interface (see below), live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.

Please select user typeResellerEnd User Preferences To receive the most relevant information, please let us know what you are interested in:

Output in terms of PAR, color mixing, and spread is all improved with the new fixtures. The inclusion of more LEDs provides a more targeted full spectrum, while TIR lenses shape the light’s output and enhance its efficiency. The result is more vibrant colors, a slightly wider output area, and higher average PAR. A combination of LED placement and lens geometry further reduces color separation.

We are working to fully integrate the pE-800 into all major third party imaging software programs, but in the meantime you can use the pE-6501-8 USB controlled TTL trigger box for software control. Tutorial videos show you how to configure cellSens and MetaMorph.

The myAI® app connects through your phone to the AI LED using Bluetooth Low Energy (BLE), allowing you to set up and program schedules and settings. The Prime HD will be replaced with the Prime 16HD, offering more color, more spread, more power, and a more refined design—all while maintaining the quality that made AI lights popular. The best part is that the new Prime and Hydras are available at the same price* as the current 26, 52, and Primes (US MRP and price are always subject to change).

The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.

Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

Redesigning the plastics in the Prime and Hydra lineup has resulted in two major benefits: better efficiency and improved aesthetics. Efficiency improvements reduce the demands on active cooling, leading to longer component life. Design enhancements thin the fixtures, reducing their overall profile and presence over your aquarium.

Sequence Runner Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out or other external hardware, and therefore requires minimal electronic hardware.